NEPA21
In Vivo & In Vitro Electroporator
In Vitro & In Vivo Transfection |
Super Electroporator NEPA21 |
High Transfection Efficiency & Viability Without Special Buffers
| ◆ Features |
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● In-Vitro Transfection
● In-Vivo Transfection
● In-Utero Transfection
● In-Ovo Transfection
● Ex-Vivo Transfection
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| *NEPA21 electroporator can cover all application range of the old-type CUY21 electroporator. |
| Request a Demo |
| See the performance of NEPA21 electroporator with your cells. |
| ◆ Novel 3-Step Multiple Electroporation Pulse | ||
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The 3-step pulse with voltage decay results in higher transfection efficiency and lower damage WITHOUT special buffers. |
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| Step 1 | Poring Pulse Mode: High Voltage, Short Duration, Multiple Pulse(s), Voltage Decay This poring pulse is for forming pores (small holes) in cell membrane with minimum damage. |
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| Step 2 | Transfer Pulse Mode: Low Voltage, Logn Duration, Multiple Pulses, Voltage Dcay. This transfer pulse is for delivering the target molecules (DNA, RNA, etc.) into cells with minimum damage.. |
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| Step 3 | Polarity Exchanged Transfer Pulse This can increase the transfection efficiency. |
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| Applicatios with Electroporation Cuvettes |
 *Please click here for Electroporation Cuvette information. |
| NEPA21 electroporator makes it possible to achieve high transfection efficiency
and viability without resourse to special buffers for primary cells, stem cell and difficult-to-transfect cells such as immune cells, blood cells, etc. |
| 1) Primary Cells |
| - BMMC Primary Mouse Bone Marrow-Derived Mast cells | |
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| Viability: 64% | Transfon Efficiency: 78% |
| FACS Data | |
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| - Primary Mouse Neurons, Cerebral Cortex | |
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| Viability: 80% | Transfon Efficiency: 70% |
| - Primary Rat Bulbar Nerve Cells | |
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| Viability: 80% | Transfon Efficiency: 75% |
| - HASM Primary Human Airway Smooth Muscle cells | |
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| Viability: 90% | Transfon Efficiency: 80% |
| - Primary Human Endometrial Stromal Cells | |
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| Viability: 95% | Transfon Efficiency: 90% |
| ◆ Disposable Kit Comparison |
| Transfection Device | NEPA21 (Nepa Gene) | N (Company L) | N (Company I) |
| Disposable Kit | Cuvettes ONLY No Special Buffers |
Transfection Kits With Special Buffers |
Transfection Kits With Special Buffers |
| Cost per One Sample *USA price |
$2.40 ($120.00/50 pcs) |
$16.00 ($160.00/10 RCT) |
$17.40 ($435.00/25 tips) |
| Is your lab still using the transfection device that requires
expensive disposable kits? We hear from a lot of researchers that they are not satisfied with its high running cost. The running cost of NEPA21 is much lower than other transfection devices! |
| 2) Cell Lines |
| V: Viability, TE: Transfection Efficiency |
| Cell Line | V | TE | Cell Line | V | TE | |||
| Species: Human | Raji | Human Burkitt's Lymphoma cells | 74% | 53% | ||||
| HeLa | Human Cervical Carcinoma cells | 95% | 95% | LCL | Human Lymphoblastoid cell | 55% | 43% | |
| 293 | Human Embryonic Kidney cells | 90% | 90% | Nalm-6 | Human B-cell Precursor Leukemia cells | 65% | 63% | |
| 293T | Human Embryonic Kidney cells | 90% | 90% | KU812 | Human basophilic leukaemia cells | 96% | 42% | |
| TIG-7 | Human Embryonic Lung Fibroblasts | 89% | 76% | MutuⅠ | Human Burkitt Lymphoma cells | 87% | 91% | |
| MRC-5 | Human Embryonic Lung Fibroblasts | 85% | 90% | MutuⅢ | Human Burkitt Lymphoma cells | 54% | 92% | |
| SUSM-1 | Human Fibroblasts | 77% | 71% | |||||
| KMST-6 | Human Fibroblasts | 70% | 60% | Species: Mouse | ||||
| HT1080 | Human Fibrosarcoma cells | 93% | 81% | MEF | Mouse Embryonic Fibroblasts | 80% | 90% | |
| MIA-PaCa-2 | Human Pancreatic Carcinoma cells | 80% | 77% | L | Mouse L cells | 90% | 65% | |
| HepG2 | Human Hepatoma cells | 80% | 76% | MC3T3-E1 | Mouse Osteoblastic cells | 40% | 80% | |
| HuH-7 | Human Hepatoma cells | 70% | 60% | MS-1 | Mouse Pancreatic Endothelial cells | 90% | 90% | |
| H1299 | Human Lung Cancer cells | 90% | 90% | RAW264.7 | Mouse Macrophage-like cells | 70% | 56% | |
| HSC-2 | Human Squamous Carcinoma cells | 93% | 98% | MIN6 | Mouse Pancreatic Beta cells | 57% | 71% | |
| HSC-3 | Human Squamous Carcinoma cells | 93% | 98% | MEL | Mouse Erythroleukemia cells | 70% | 50% | |
| OVCAR-3 | Human Ovarian Carcinoma cells | 90% | 79% | XS106 | Mouse Dendritic cells | 61% | 45% | |
| RMG-1 | Human Ovarian Carcinoma cells | 97% | 67% | mDC | Mouse Myeloid Dendritic cells | 79% | 72% | |
| TE-1 | Human Esophageal Carcinoma cells | 85% | 41% | MC/9 | Mouse Mast cells | 76% | 84% | |
| TE-8 | Human Esophageal Carcinoma cells | 85% | 40% | TS | Mouse Trophoblast Stem cells | 59% | 47% | |
| MCF-7 | Human Breast Cancer cells | 90% | 93% | |||||
| T47D | Human Breast Cancer cells | 90% | 85% | Species: Rat | ||||
| NUGC-3 | Human Gastric Carcinoma cells | 73% | 68% | PC12 | Rat Adrenal Pheochromocytoma cells | 70% | 50% | |
| A549 | Human Lung Adenocarcinoma cells | 85% | 90% | H9c2 | Rat Ventricular Myoblasts | 71% | 82% | |
| LNCaP | Human Prostate Carcinoma cells | 71% | 90% | REF | Rat Embryonic Fibroblasts | 90% | 99% | |
| RKO | Human Colorectal Carcinoma cells | 80% | 40% | C6 | Rat Glioma cells | 80% | 67% | |
| SK-N-SH | Human Neuroblastoma cells | 95% | 95% | |||||
| SH-SY5Y | Human Neuroblastoma cells | 60% | 90% | Species: Hamster | ||||
| KG-1-C | Human Oligodendroglial cells | 85% | 60% | CHO | Chinese Hamster Ovary cells | 74% | 90% | |
| HaCaT | Human Keratinocyte cells | 40% | 80% | CHO-K1 | Chinese Hamster Ovary cells | 95% | 95% | |
| CCD1079sk | Human Skin Fibroblasts | 40% | 53% | |||||
| iHAM-4 | Human Amniotic Mesenchymal cells | 59% | 95% | Species: Chicken | ||||
| iHAM-7 | Human Amniotic Epithelial cells | 70% | 40% | DT40 | Chicken B cells | 38% | 40% | |
| HTR-8/Svneo | Human Trophoblast cells | 95% | 67% | |||||
| Jurkat | Human T-cell Leukemia cells | 71% | 73% | Species: Carp | ||||
| Namalwa | Human Burkitt's Lymphoma cells | 70% | 75% | GTS9 | Ginbuna Thymus cells | 50% | 30% | |
| Applications with Adherent Cell Electrodes |
| By using Adherent Cell Electrode CUY900 series, now it is possible to transfer DNA/RNA directly into cells in adherence in a commercially available multi-well plate. |
| ● Gene transfection into primary neurons (adherent) by electroporation | |
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| pCAGGS-EGFP plasmid was transferred into primary neurons cultured for 6 days in adherent state. The neurons were prepared from E15 mouse cerebral cortex. |
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| A): 2 steps pulse electroporation using the electrodes for adherent cells
(CUY900-13-3-5) B): EGFP fluorescence image of the neurons 2 days after electroporation C): High magnification image of Figure B Many robust EGFP signals suggest high transfection efficiency. D): High magnification image of Figure C (x40) Neurites are shown clearly. |
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| Department of Neurochemistry, National Institute of Neuroscience | |
| Applications with In-Vivo Electrodes |
| NEPA21 electroporator and CUY series in-vivo electrodes make it possible to transfer DNA/RNA into mouse/rat muscle, skin, liver, kidney, testis, ovary, brain, retina, cornea, etc. |
| ● Transfection into brain, eye, muscle, skin, liver, kidney, testis, etc. |
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| Muscle | Skin | Testis | Retina | Brain | Egg |
| *Please click here for detailed application information. |
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| *Please cick here for electrode information. | |||||
| Applications with In-Utero / Exo-Utero Electrodes |
| NEPA21 electroporator and CUY series in-utero electrodes make it possible to transfer DNA/RNA into mouse/rat embryo (Cerebral Cortex, Hippocampus, Spinal Cord, etc.) |
| ● Transfection into mouse/rat embryonic brains | |||||
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| *Please click here for detailed application information. |
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| *Please click here for electrode information. |
| Applications with In-Ovo Electrodes |
| ● Transfection into chick embryos | |
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| Transfection into the central canal | Transfection into the somites (A, E), hematopoietic system (B, F), notochord (C, G), and lateral plate mesoderm (D, H) |
| *Please click here for detailed application information. |
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| *Please click here for electrode information. | |
| Applications with Ex-Vivo Electrodes |
| ● Transfection into brain tissues | |
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| Transfection into the brain slice. | |
| *Please click here for detailed application information. |
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| *Please click here for electrode information. | |
| ● Transfection into embryos in the whole embryo culture system | |||||
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| Transfection into the developing rat cortex | Transfection into the rat spinal cord | ||||
| *Please click here for detailed application information. |
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| *Please click here for electrode information. | |||||
| *All features and specifications subject to change without notice. |
